Midi GeBaflex-tube Vol. 50 - 800 µl, gel slice size for electro elution: 0.5 x 1cm
Maxi GeBAflex-tube Vol. 100 - 3000 µl, gel slice size for electro elution: 1 x 2 cm
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Product
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Unit
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MWCO
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Catalog
|
|
GeBAflex-tube
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5
|
3500
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TO30
|
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GeBAflex-tube
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15
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3500
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TO35
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Dialysis process
Dialysis Dialysis float
Midi GeBAflex-tube: Gel slice size: 0.5 x 1.1cm Maximum volume: 800 µl
Maxi GeBAflex-tube: Gel slice size: 1 x 2cm Maximum Volume: 3000 µl
Using the GeBAflex-tube for the extraction of the Oligo from the gel (PAGE purification), by electro elution, the process of extraction is reduced to just 15-20 min. This process is simple, straight forward, and extremely time efficient. GeBAflex-tube recovers any oligo ranging from 15 nt to 150 nt with a 95% Oligo recovery. Following oligo PAGE purification, a desalting step is often required. Here are two short alternative methods for desalting:
Applied Biosystems Voyager System 1171
T:DNAMass Spec DataARK3-100_B11_0001.dat
Sample Description: , Comments: Israels Samples
Acquired: 19:30:00, MarcPrinted: 19:30, March 24, 2004
Mode of operation: Linear
Extraction mode: Delayed
Polarity: Positive
Acquisition control: Automatic
Accelerating voltage: 20000 V
Grid voltage: 94%
Guide wire 0: 0.07%
Extraction delay time: 200 nsec
Acquisition mass range: 2500 -- 10000 Da
Number of laser shots: 50/spectrum
Laser intensity: 2400
Laser Rep Rate: 20.0 Hz
Calibration type: External -- D:calibration8k and less 3-08-04.cal
Calibration matrix: 3-Hydroxypicolinic acid
Low mass gate: 2400 Da
Digitizer start time: 31.78
Bin size: 10 nsec
Number of data points: 3158
Vertical scale: 1000 mV
Vertical offset: 0%
Input bandwidth: 150 MHz
Laser control: Automated
Sample positioning: Automated
Search pattern file: C:VOYAGER400well.sp
Auto storage mode: Accumulate all passing
Min intensity: 1000
Max intensity: 0
Resolution: 0
Signal-to-noise: 80
Sample well: B11
Plate ID: 400-5
Serial number: 1171
Instrument name: Voyager-DE
Plate type filename: C:VOYAGER400 well plate.plt
Lab name: PE Biosystems
Absolute x-position: 25655.5
Absolute y-position: 39025.2
Relative x-position: -144.566
Relative y-posititon: 268.001
Shots in spectrum: 50
Source pressure: 3.803e-007
Mirror pressure: 0
TC2 pressure: 0.01607
TIS gate width: 30
TIS flight length: 940
2499.0 3999.8 5500.6 7001.4 8502.2 10003.0
Mass (m/z)
0
1.1E+4
0
10
20
30
40
50
60
70
80
90
100
% Intensity
Voyager Spec #1=>MC=>BC=>NF0.7[BP = 6104.4, 11433]
6103.77
3054.08
6197.19 4644.93
- Using the same GeBAflex-tube, after electro elution, can be used directly for dialysis process (80-100 min).
- Using column for desalting.
Oligo manufacturers require high throughput samples PAGE purification process for quantities ranging from 10 to 1000 samples a day, GeBAflex-tube fit into most standard horizontal electrophoresis unit, typical MAXI horizontal electrophoresis unit can accommodate multiple number of electro elution GeBAflex-tubes (see pictures).
The similar process can be also applied to peptide purification, for above 30 amino acide.
GeBAflex-tube 30 min oligo PAGE purification protocol, secure & elegant. A full solution which easily integrate into existing production process.
