Introduction:

Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for the separation and estimation of the molecular weight of individual proteins.

The accuracy of molecular weight determination & characterization is limited by protein low recovery. Recently, MALDI-TOF-MS has found widespread use for the determination of molecular mass of intact proteins isolated from gels. Isolation of proteins from gels with the newly developed GeBAflex-tube electro-elution system delivers over 80% recovery yields. A combination of SDS-PAGE, GeBAflex-tube electro-elution system, and MS buffer removing SDS (included in kit), delivers MALDI-TOF-MS,  and greater accuracy in the determination of protein molecules. Now we can analyze low yield and complex proteins, such as integral membrane proteins (hydrophobic) or high molecular masses protein.

PRODUCT CATALOG  REFERENCE

Purity: No contamination with Protease, RNase, DNase and PCR free.
Membrane: ultra-clean, sulfur and heavy metal free, and EDTA treated.
Single use unit, no need for cleaning and autoclave. Single step process.    

A Maxi GeBAflex-tube inside Maxi-supporting tray.      

 High throughput electro elution with GeBAflex-tube using standard electrophoresis unit.
Picture shows 24 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80mïn.

Picture shows 4 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80mïn.

 Mini GeBaflex-tube   Vol.  10 - 250µl,    Max. gel slice size for electro elution: 0.4 x 1cm

Mini support plate: High x width between electrodes =  45 x 75mm, minimum depth 14mm  

Midi GeBaflex-tube   Vol.  50 - 800µl, Max. gel slice size for electro elution: 0.5 x 1cm   Midi support plate for 4 midi GeBA  flex-tube                                                                                High x width between electrodes =  67 x 111mm, minimum depth 24mm 

Maxi GeBaflex-tube  Vol 100 - 3000 µl,   Max. gel slice size for electro elution: 1 x 2 cm

Maxi support plate for 3 maxi GeBAflex-tube:                          High x Width between electrodes =  92 x 113 mm, minimum depth 28mm 

This unique method provides a powerful means for characterizing endogenous proteins in a wide molecular weight range separated by SDS-PAGE. The combination of Geba-flex tube electro-elution, SDS-Page, and MS Buffer provide a significantly improved protein yield and SDS free samples. The end result is a MALDI-MS analysis with greater sensitivity. The GeBA flex-tube tool provides high protein yield recovery and the MS buffer contained in the GeBA flex-tube kit cleans SDS thoroughly.