Introduction:

Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for the separation and estimation of the molecular weight of individual proteins

Accuracy of molecular weight determination & characterization is limited by protein low recovery. Recently MALDI-TOF-MS has found widespread use for the determination of molecular mass of intact proteins isolated from gels. Isolation of proteins from gels with the newly developed GeBAflex-tube electro-elution system delivers over 80% recovery yields. Combination of SDS-PAGE, GeBAflex-tube electro-elution system, and MS buffer removing SDS (included in kit), delivers MALDI-TOF-MS,  greater accuracy determination of protein molecular. Now we can analyze low & difficult protein, such as integral membrane proteins (hydrophobic) or high molecular masses protein.

The unique method provides a powerful means for characterizing endogenous proteins of wide molecular weight range separated by SDS-PAGE. The combinations of the three methods provide significantly improve protein yield and SDS free samples. The end result is a MALDI-MS analysis with greater sensitivity. GeBAflex-tube tool provide high protein yield recovery, MS buffer contained in GeBAflex-tube kit clean SDS thoroughly.

PRODUCT CATALOG  REFERENCE

Purity: No contamination with Protease, RNase, DNase and PCR product free.
Membrane: ultra-clean, sulfur and heavy metal free and EDTA treated.
Single use unit, no need for cleaning and autoclave. Single step process.    

A Maxi GeBAflex-tube inside Maxi-supporting tray.      

 High throughput electro elution with GeBAflex-tube using standard electrophorese unit.
Picture show 24 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80mïn.

Picture show 4 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80mïn.

 Mini GeBaflex-tube   Vol.  10-250µl,    Max. gel slice size for electro elution: 0.4x1cm

Mini support plate: High x width between electrodes =  45 x 75mm, minimum depth 14mm  

Midi GeBaflex-tube   Vol.  50-800µl,   Max. gel slice size for electro elution: 0.5x1cm                                           Midi support plate for 4 midi GeBAflex-tube :                                                                                  High x width between electrodes =  67 x 111mm, minimum depth 24mm 

Maxi GeBaflex-tube  Vol 100-3000µl,   Max. gel slice size for electro elution: 1x2cm

Maxi support plate for 3 maxiGeBAflex-tube:                                                                                                                                        High x Width between electrodes =  92 x 113 mm, minimum depth 28mm